Summary:
A Singapore institute of higher learning has developed a novel rapid multiplex meat speciation kit to identify meat products authenticity, which is as an important issue in food regulatory control. This developed kit uses polymerase chain reaction amplification in the presence of double stranded DNA binding dye followed by high resolution melting technology. The technology provider is interested in license agreements or commercial agreements with technical assistance with MNEs/SMEs of all sizes.
Description:
A rapid, cost-effective examination of meat adulteration is a very critical issue in food regulatory control. Determining the fraudulent replacement of higher commercial valued meat species by inferior, cheaper or undesirable alternatives is necessary for ethical requirements, specific food allergies, religious affairs, fraud, and malicious marketing practices in addition to safety, economic, religious (Halal testing) and legal concerns.
The technology provider's rapid multiplex quantitative assay for meat speciation including beef, pork, chicken, sheep, dog and horse as well as meat products and processed meat such as sausage, meatball, etc., is novel and has multiple advantages over currently used meat identification assays.
The Rapid Multiplex Meat Speciation kit developed employs polymerase chain reaction (PCR) amplification in the presence of double stranded DNA binding dye followed by high resolution melting (HRM) technology. Detection of meat species is based on the pre-determined melting point of PCR products. For example, a PCR product which melts at 70 degrees indicates it is from beef and at 74 degrees from pork.
The Rapid Multiplex Meat Speciation kit comprises of the following technologies:
- Primer Design
- Multiplex PCR optimisation
- Optimisation of Melting Point of PCR products so that they can be distinguished from each other
- Innovative method of detection based on the pre-determined melting point of PCR products
Type (e.g. company, R&D institution…), field of industry and Role of Partner Sought:
The Singapore institute of higher learning is interested in the following types of partnerships with MNEs or SMEs of all sizes:
i) Licensing agreement - The partner could license the technology for further development and introduce it to its customers.
ii) Commercial agreement with technical assistance - The partner could utilise the technology with technical support from the technology provider.
Stage of Development:
Project already started
IPR Status:
Secret Know-how
External code:
TOSG20210125004
